Search results for "Two-photon excitation microscopy"

4D (x-y-z-t) imaging of thick biological samples by means of Two-Photon inverted Selective Plane Illumination Microscopy (2PE-iSPIM)

2015

AbstractIn the last decade light sheet fluorescence microscopy techniques, such as selective plane illumination microscopy (SPIM), has become a well established method for developmental biology. However, conventional SPIM architectures hardly permit imaging of certain tissues since the common sample mounting procedure, based on gel embedding, could interfere with the sample morphology. In this work we propose an inverted selective plane microscopy system (iSPIM), based on non-linear excitation, suitable for 3D tissue imaging. First, the iSPIM architecture provides flexibility on the sample mounting, getting rid of the gel-based mounting typical of conventional SPIM, permitting 3D imaging of…

Enhanced network activity despite clinical recovery in experimental neuroinflammation using two-photon calcium imaging

2014

Self-trapped exciton luminescence in crystalline α-quartz under two-photon laser excitation

2003

Abstract The luminescence of pure crystalline α-quartz is studied under pulsed ArF laser excitation. The luminescence parameters obtained correspond well with those of self-trapped excitons (STEs) in α-quartz, indicating that the excitation process is two-photon. The efficiency of two-photon excitation is of the same order of magnitude as the one-photon excitation of sodium salicylate. The STE luminescence decay kinetics and their temperature dependence under photoexcitation were recorded with higher accuracy than previously. Changes in the decay kinetics with temperature are explained by the splitting of the STE triplet state in zero magnetic field and are analyzed with the assumption of t…

Patterning and tuning of electrical and optical properties of graphene by laser induced two-photon oxidation

2015

Graphene, being an ultrathin, durable, flexible, transparent material with superior conductivity and unusual optical properties, promises many novel applications in electronics, photonics and optoelectronics. For applications in electronics, patterning and modification of electrical properties is very desirable since pristine graphene has no band gap. Here we demonstrate a simple all-optical patterning method for graphene, based on laser induced two-photon oxidation. By tuning the intensity of irradiation and the number of pulses the level of oxidation can be controlled to high precision and, therefore, a band gap can be introduced and electrical and optical properties can be continuously t…

Quantum effects in the dynamics of intensity-dependent two-mode two-photon models of radiation—matter interaction

1996

Abstract We study the two-photon interaction of a two-state localized system with two modes of a quantized electromagnetic or elastic field. Assuming the coupling strength and the atom-field detuning depending on the mode populations, we find that the quantum atomic dynamics manifests nonclassical features related to the specific nonlinear model investigated.

Squeezing in a two-photon Dicke hamiltonian

1986

Abstract The single-mode, two-level atom Dicke hamiltonian with two-photon atom-field coupling is treated exactly and it is shown to yield a certain degree of squeezing in the field variables. This result is briefly discussed in connection with the previously shown absence of squeezing in the two-photon laser model.

Nonlinear dynamics of a two-photon Fabry–Pérot laser

2000

Abstract The steady-state emission, stability and temporal dynamics of a single-mode two-photon laser with a Fabry–Perot cavity is investigated and compared with that of a ring-cavity laser. It is found that the Fabry–Perot cavity makes the laser less efficient than the ring cavity because of spatial hole burning, but the domain of stability is larger for the Fabry–Perot laser. The intensity and phase dynamics are numerically investigated and distinctive features are found in the phase dynamics as compared with one-photon lasers.

Two-Photon Transitions in CW-ENDOR Spectra of Radicals in Fluid Solution

2002

The radical anion of duroquinone (2,3,5,6-tetramethyl-1,4-benzoquinone) was used as a model compound to describe the low-frequency (LF) ENDOR lines observed in many radical systems. These LF lines ...

Optical-sectioning improvement in two-color excitation scanning microscopy

2004

We present a new beam-shaping technique for two-color excitation fluorescence microscopy. We show that by simply inserting a properly designed shaded-ring filter in the illumination beam of smaller wavelength, it is possible to improve the effective optical sectioning capacity of such microscopes by 23%. Such an improvement is obtained at the expense of only a very small increasing of the overall energy in the point-spread-function sidelobes. The performance of this technique is illustrated by a numerical imaging simulation.

Line shape measurements of rubidium 5S-7S two-photon transition

2014

We report the use of a digital lock to measure the line profile and center frequency of rubidium 5S-7S two-photon transitions with a cw laser referenced to an optical frequency comb. The narrow, two-photon transition, 5S-7S (760 nm), insensitive to first-order in a magnetic field, is a promising candidate for frequency reference.